Inhibition of the Growth and Development of Potato Early Blight Pathogen (Alternaria solani) by Combining Penicillium chrysogenum VKM F-4876D with Some Strobilurin-, Triazole-, and Phenylpyrrole-Based Fungicides

The biological control of fungal crop diseases based on the use micro-organisms or their metabolites is a promising environmentally friendly alternative to common fungicide-based technologies. Penicillium fungi which synthesize various biologically active compounds with wide range antimicrobial activity are special interest as potential producers antifungal biopreparations. purpose this study was evaluation dry biomass chrysogenum VKPM F-4876D strain (DMP), both individually and combined commercial azoxystrobin-, fludioxonil-, difenoconazole-, tebuconazole-based fungicides commonly used early blight, towards Alternaria solani, an infectious agent potato disease. performed under in vitro (cultivation solid liquid media) vivo (detached leaves) conditions. cultivation A. solani agarized medium supplemented 5 g/L DMP caused significant morphological changes pathogen colonies, whereas 7.5 resulted complete suppression development. addition culture significantly increased electrolyte release, i.e., impaired cell membrane integrity pathogen. Combining (1–2 g/L) at dose 0.01–10 mg/L growth inhibition solani. best result obtained for combination (1 fludioxonil (1–5 mg/L) tebuconazole (10 mg/L): prolonged effect (96–97% inhibition) observed during whole 14-day period observation. treatment detached leaves 1 prior artificial infection four-fold increase number uninfected two-fold reduction severely infected compared control. These results demonstrate possibility develop efficient approach manage blight provide prerequisites pesticide load agrobiocoenoses, thus contributing restoration ecological balance prevention emergence spread resistant strains.